From whole specimens to histological level reconstructions, images derived from 3D synchrotron-based microtomography (SR-microCT) have emerged as a tool that is revolutionizing a wide range of biological research. This paper presents the first commissioning results from experiments to image the anatomy of small animals and specimens from zooarchaeology and palaeontology carried out on MOGNO, the nano- and microtomography beamline at Sirius/LNLS, Brazil.